| 产品中文名称 | 重组人Noggin蛋白，His-标签（Noggin） |
| 产品英文名称 | 重组人Noggin蛋白，His-标签（Noggin） |
| 产品别名 | Nog |
| 产品描述 | The secreted polypeptide Noggin, encoded by the Noggin gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, Noggin may have a principal role in creating morphogenic gradients. Noggin appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of Noggin suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human Noggin mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as Noggin. All Noggin mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of human Noggin is highly homologous to that of Xenopus, rat and mouse. |
| 表达宿主 | 大肠杆菌 |
| 种属 | 人 |
| 序列 | 氨基酸序列来源于：人源Noggin (Q13253) (Gln28-Cys232) 表达的蛋白片段。 |
| 活性 | 通过其抑制BMP 4蛋白诱导ATDC 5细胞产生碱性磷酸酶的能力来测量。这种作用的ED 50浓度为1.22 ng/mL。 |
| 蛋白长度 | 重组人Noggin由206个氨基酸组成，预测分子量为23.2 KD。 |
| 纯度 | > 98 % ，使用SDS-PAGE检测 |
| 特点&优势 | 通过LAL法测定，每微克蛋白里内毒素含量<0.1 EU。 |
| 产品形式 | 冻干粉，冻干缓冲液为无菌的20 mM Tris，50 mM NaCl，pH 8.0。 |
| 基因ID | 25317 |
| 蛋白序列链接 | NOG - Noggin - Homo sapiens (Human) | UniProtKB | UniProt |
| 使用中注意事项 | 开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶，且浓度不低于102 µg/mL。客户可根据后续的实验需求，进行进一步的稀释。 |
| 保存建议 | 本产品为冻干粉形式，建议：将冻干粉产品保存于-20℃以下的干燥环境中。本产品溶解之后，可在4℃保存2-7天，长期使用请置于-20℃以下。为长期保存，推荐添加载体蛋白（0.1% HSA或者BSA），并酌情分装保存，避免反复冻融。 |
| 运输条件 | 冰袋运输（蓝冰） |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |